1 Weiler14 Ex2R18C1

Putative synapse locations were obtained by Anish Simhal.
Around each synapse across each channel the sum of pixel intensities within an 11x11x11 cube are calculated to produce a feature vector. These feature vectors are combined into a data matrix. We replace the DAPI channels with their first principal component. The data matrix is then z-scored before running it through meda.

We did this for samples of size 1,000 and 10,000.

1.1 Sample size 1,000

1.1.1 1-d Heatmap

1.1.2 Location plots

1.1.3 Outliers as given by randomForest

1.1.4 Correlation Matrix

1.1.5 Cumulative Variance with Elbows

1.1.6 Paired Hex-binned plot

1.1.7 Hierarchical GMM Classifications

1.1.8 Hierarchical GMM Dendrogram

1.1.9 Stacked Cluster Means plot

1.1.10 Cluster Means

1.2 Sample size 10,000

1.2.1 1-d Heatmap

1.2.2 Location plots

1.2.3 Outliers as given by randomForest

1.2.4 Correlation Matrix

1.2.5 Cumulative Variance with Elbows

1.2.6 Paired Hex-binned plot

1.2.7 Hierarchical GMM Classifications

1.2.8 Hierarchical GMM Dendrogram

1.2.9 Stacked Cluster Means plot

1.2.10 Cluster Means